Course
MC 208 (AUG) 3:0
Principles of Genetic Engineering
Growth and maintenance of bacterio-phages and bacterial strains containing plasmids. Enzymes used in genetic engineering. Vectors used in molecular cloning and expression of genes, promoter analyses, and gene targeting in bacterial, mammalian, human, and plant systems. DNA, RNA, and protein isolation, purification, and fractionation methods. Radioactive and nonradioactive labelling of nucleic acids and proteins, and detection. Nucleic acids hybridisation methods. Transformation and transfection methods. Gene and cDNA cloning methods.In vitro genome packaging systems and construction of genomic DNA and cDNA libraries.Detection and characterisation methods for genes and chromosomes. Nucleic acids sequencing methods.Methods for protein analysis, protein-nucleic acid, and protein-protein interactions. Site-specific mutagenesisin vitro and in vivo.Random mutagenesis methods in vitro and in vivo. Genome engineering methods. Polymerase chain reaction (qualitative and quantitative), methods, and applications.Antisense technology and RNA silencing techniques. DNA and Protein microarrays. Methods to generate transgenic animals. Applications of Genetic Engineering Methods in Medicine and Agriculture.
References
- J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, 3rdEdn: Vol. I, II, & III, Cold Spring Harbor Laboratory Press.
- J. J. Greene and V. B. Rao. Recombinant DNA Principles and Methodologies. CRC Press.
- S. B. Primrose and R. M. Twyman. Principles of Gene Manipulation and Genomics, 7thEdn, Blackwell Publishing.
- Original papers describing the principles.